BIO 688 Methods in Chemical Biology
A discussion of the theories underlying various techniques of molecular biology which are used in the biotechnology industry. Topics include all recombinant DNA techniques; DNA isolation and analysis; library construction and screening; cloning; DNA sequencing; hybridization and other detection methods; RNA isolation and analysis; protein isolation and analysis (immunoassay, ELISA, etc.); transgenic and ES cell methods; electrophoresis (agarose, acrylamide, two dimensional, and SDS-PAGE); column chromatography; and basic cell culture including transfection and expression systems.
Prerequisite
BIO 381 or Graduate Students only